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KMID : 0921620190490030124
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Journal of Bacteriology and Virology
2019 Volume.49 No. 3 p.124 ~ p.132
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Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties
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Yang Dong-Kun
Kim Ha-Hyun
Lee Eun-Jin
Yoo Jae-Young
Yoon Soon-Seek
Park Jung-Won
Kim Chae-Hyun
Kim Ho-Ryoung
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Abstract
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Canine adenovirus type 1 (CAV-1) infection results in hepatitis in dogs. In this study, we investigated the biologic and genetic characteristics of the CAV-1 vaccine strain (CAV1V) to improve quality control about CAV vaccine. The identity of CAV1V as CAV-1 was confirmed based on its cytopathic effects and the results of hemagglutination (HA) and immunofluorescence assays, and electron microscopy. The CAV1V strain reached 107.5 TCID50/mL in MDCK cells at 4 days post-inoculation and exhibited hemmagglutination activity of 256 U using guinea pig erythrocytes. Intranuclear fluorescence in the infected cells was observed and typical adenoviruses were observed in electon microscope. CAV1V strain was identified as a CAV-1 strain by nucleotide sequence analysis. In a comparison of the nucleotide sequences of the fiber genes of several CAV strains, CAV1V showed the highest similarity (99.8%) with the GLAXO strain, which was isolated in Canada. Our biological characterization of CAV1V will facilitate quality control of the canine hepatitis vaccine.
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KEYWORD
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Canine adenovirus type 1, Genetic characterization, Vaccine
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